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Descripción
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| The bacterial pathogen Pseudomonas syringae pv. tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defense-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His6-tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of Photosystem II (PSII), as an interacting protein. HopN1 localized to onion cell plastids and both degraded PsbQ and inhibited PSII activity in tomato thylakoid preparations, whereas a HopN1D299A non-catalytic mutant lost these abilities. Virus-induced gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant defense and a target for pathogen suppression. | |
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Internacional
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Si |
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ISSN o ISBN
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Entidad relacionada
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8th International Conference on Pseudomonas syringae and related pathogens |
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Nacionalidad Entidad
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Sin nacionalidad |
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Lugar del congreso
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Oxford- UK |