Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments
Año:2012
Áreas de investigación
  • Biología molecular, celular y genética,
  • Entomología,
  • Agricultura,
  • Medio de producción agrícola,
  • Insecticida
Datos
Descripción
BACKGROUND: Knowledge of pesticide selectivity to natural enemies is necessary for a successful implementation of biological and chemical control methods in integrated pest management (IPM) programs. Diacylhydrazine (DAH)-based ecdysone agonists also known as molting-accelerating compounds (MACs) are considered a selective group of insecticides, and their compatibility with predatory Heteroptera, which are used as biological control agents, is known. However, their molecular mode of action has not been explored in beneficial insects such as Orius laevigatus (Fieber) (Hemiptera: Anthocoridae). RESULTS: In this project in vivo toxicity assays demonstrated that the DAH-based RH-5849, tebufenozide and methoxyfenozide have no toxic effect against O. laevigatus. The ligand-binding domain (LBD) of the ecdysone receptor (EcR) of O. laevigatus was sequenced and a homology protein model was constructed which confirmed a cavity structure with 12 ?-helixes, harboring the natural insect molting hormone 20-hydroxyecdysone. However, docking studies showed that a steric clash occurred for the DAH-based insecticides due to a restricted extent of the ligand-binding cavity of the EcR of O. laevigatus. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for O. laevigatus. The modeling/docking experiments are indications that these pesticides do not bind with the LBD-EcR of O. laevigatus and support that they show no biological effects in the predatory bug. These data help in explaining the compatible use of MACs together with predatory bugs in IPM programs. Keywords: Orius laevigatus, selectivity, diacylhydrazine insecticides, ecdysone receptor, homology modelling, docking studies.
Internacional
Si
JCR del ISI
Si
Título de la revista
Pest Management Science
ISSN
1526-4998
Factor de impacto JCR
2,251
Información de impacto
Volumen
18
DOI
Número de revista
2
Desde la página
1586
Hasta la página
1594
Mes
JULIO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Fermín Amor Parrilla (UPM)
  • Autor: Olivier Christiaens (Ghent University)
  • Autor: Paloma Bengochea Budia (UPM)
  • Autor: Maria Pilar Medina Velez (UPM)
  • Autor: Pierre Rougé (Université Paul Sabatier)
  • Autor: Elisa Viñuela Sandoval (UPM)
  • Autor: Guy Smagghe (Ghent University)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Manejo Integrado de Plagas
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